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kat2a primary cell based screening assays cell culture ht1080 human fibrosarcoma cell line ht1080  (ATCC)


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    ATCC kat2a primary cell based screening assays cell culture ht1080 human fibrosarcoma cell line ht1080
    Kat2a Primary Cell Based Screening Assays Cell Culture Ht1080 Human Fibrosarcoma Cell Line Ht1080, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 4054 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ht1080+fibrosarcoma+cells/HT-1080/us12655105-1783-0-16
    Average 98 stars, based on 4054 article reviews
    kat2a primary cell based screening assays cell culture ht1080 human fibrosarcoma cell line ht1080 - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Cell Culture:

    Article Title: A Facile Method Based on Faster R-CNN for Cell Detection in Microfluidic Devices.
    Article Snippet: K-562 lymphoblast cell and T cell lines were cultured in RPMI-1640 media (ATCC) supplemented with 10% fetal bovine serum (FBS, VWR). .. HT1080 fibrosarcoma cells were cultured in DMEM medium (ATCC) supplemented with 10% fetal bovine serum (FBS, VWR). ..

    Article Title: Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal Therapy
    Article Snippet: .. Cell Culture, Viability Assay, and Cell Labeling: The HT1080 fibrosarcoma cells, obtained from the American Type Culture Collection (ATCC), were transfected to overexpress human FAP (hFAP) as previously described. .. [39] FAP expression was confirmed by flow cytometry. hFAP cells were cultured in DMEM STINO medium, consisting of Dulbecco’s Modified Eagle Medium (DMEM, 4.5 g L 1 D-Glucose, without Sodium Pyruvate, Gibco) supplemented with 10% fetal bovine serum (Biochrom), 1% streptomycin and penicillin (Biochrom), and 1% MEM non-essential amino acid solution (Sigma–Aldrich) at 37 °C with 5% CO2, and the medium was changed every 2–3 days.

    Article Title: A Facile Method Based on Faster R‑CNN for Cell Detection in Microfluidic Devices
    Article Snippet: K-562 lymphoblast cell and T cell lines were cultured in RPMI-1640 media (ATCC) supplemented with 10% fetal bovine serum (FBS, VWR). .. HT1080 fibrosarcoma cells were cultured in DMEM medium (ATCC) supplemented with 10% fetal bovine serum (FBS, VWR). ..

    Viability Assay:

    Article Title: Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal Therapy
    Article Snippet: .. Cell Culture, Viability Assay, and Cell Labeling: The HT1080 fibrosarcoma cells, obtained from the American Type Culture Collection (ATCC), were transfected to overexpress human FAP (hFAP) as previously described. .. [39] FAP expression was confirmed by flow cytometry. hFAP cells were cultured in DMEM STINO medium, consisting of Dulbecco’s Modified Eagle Medium (DMEM, 4.5 g L 1 D-Glucose, without Sodium Pyruvate, Gibco) supplemented with 10% fetal bovine serum (Biochrom), 1% streptomycin and penicillin (Biochrom), and 1% MEM non-essential amino acid solution (Sigma–Aldrich) at 37 °C with 5% CO2, and the medium was changed every 2–3 days.

    Labeling:

    Article Title: Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal Therapy
    Article Snippet: .. Cell Culture, Viability Assay, and Cell Labeling: The HT1080 fibrosarcoma cells, obtained from the American Type Culture Collection (ATCC), were transfected to overexpress human FAP (hFAP) as previously described. .. [39] FAP expression was confirmed by flow cytometry. hFAP cells were cultured in DMEM STINO medium, consisting of Dulbecco’s Modified Eagle Medium (DMEM, 4.5 g L 1 D-Glucose, without Sodium Pyruvate, Gibco) supplemented with 10% fetal bovine serum (Biochrom), 1% streptomycin and penicillin (Biochrom), and 1% MEM non-essential amino acid solution (Sigma–Aldrich) at 37 °C with 5% CO2, and the medium was changed every 2–3 days.

    Transfection:

    Article Title: Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal Therapy
    Article Snippet: .. Cell Culture, Viability Assay, and Cell Labeling: The HT1080 fibrosarcoma cells, obtained from the American Type Culture Collection (ATCC), were transfected to overexpress human FAP (hFAP) as previously described. .. [39] FAP expression was confirmed by flow cytometry. hFAP cells were cultured in DMEM STINO medium, consisting of Dulbecco’s Modified Eagle Medium (DMEM, 4.5 g L 1 D-Glucose, without Sodium Pyruvate, Gibco) supplemented with 10% fetal bovine serum (Biochrom), 1% streptomycin and penicillin (Biochrom), and 1% MEM non-essential amino acid solution (Sigma–Aldrich) at 37 °C with 5% CO2, and the medium was changed every 2–3 days.

    Article Title: Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal Therapy
    Article Snippet: .. The HT1080 fibrosarcoma cells, obtained from the American Type Culture Collection (ATCC), were transfected to overexpress human FAP (hFAP) as previously described. .. [ ] FAP expression was confirmed by flow cytometry. hFAP cells were cultured in DMEM STINO medium, consisting of Dulbecco's Modified Eagle Medium (DMEM, 4.5 g L −1 D‐Glucose, without Sodium Pyruvate, Gibco) supplemented with 10% fetal bovine serum (Biochrom), 1% streptomycin and penicillin (Biochrom), and 1% MEM non‐essential amino acid solution (Sigma–Aldrich) at 37 °C with 5% CO 2 , and the medium was changed every 2–3 days.

    Modification:

    Article Title: Anionic Lipid Trafficking and Cell Mechanics Regulate Membrane Electrical Potential in Non-Excitable Tissue Cells
    Article Snippet: .. NIH 3T3 fibroblasts and HT1080 fibrosarcoma cells were obtained from ATCC and maintained in Dulbecco’s Modified Eagle Medium (DMEM; Corning), supplemented with 10% fetal bovine serum (FBS; Sigma) and 1% antibiotic solution containing 10,000 units/mL penicillin and 10,000 μ g/mL streptomycin (Gibco). ..

    Article Title: G2B: an optimized lentiviral vector with enhanced titer and β-globin expression for improved β-thalassemia gene therapy
    Article Snippet: .. HT1080 fibrosarcoma cells and HEK-293T producer cells (ATCC, Manassas, Virginia) were maintained in Dulbecco's Modified Eagle Medium (DMEM) (Gibco [part of Thermo Fisher] , Waltham, Massachusetts) supplemented with 10% fetal bovine serum (FBS; Gibco [part of Thermo Fisher]). .. HUDEP2 erythroid progenitors, kindly provided by Dr R. Kurita, were expanded in StemSpan SFEM II (Stemcell Technologies, Vancouver, British Columbia, Canada) containing 50 ng/mL human stem-cell factor, 3 U/mL erythropoietin (EPO), 1 μM dexamethasone, and 10 μg/mL doxycycline.

    Biomarker Discovery:

    Article Title: Development of label-free cell tracking for discrimination of the heterogeneous mesenchymal migration
    Article Snippet: .. We used HT1080 fibrosarcoma cells (ATCC CCL-121) as a cancer cell model and 3T3-Swiss fibroblasts (JCRB 9019) as a non-malignant normal cell model for the validation of our tool. .. Cells were cultured in a humidity-controlled environment under 5% CO 2 at 37 °C, using Minimum Essential Medium α (Gibco) supplemented with 10% fetal bovine serum (HyClone), 100 units/mL penicillin, and 100 μg/mL streptomycin (FUJIFILM Wako Pure Chemical Corporation).

    Article Title: Development of label-free cell tracking for discrimination of the heterogeneous mesenchymal migration.
    Article Snippet: .. We used HT1080 fibrosarcoma cells (ATCC CCL-121) as a cancer cell model and 3T3-Swiss fibroblasts (JCRB 9019) as a non-malignant normal cell model for the validation of our tool. .. Cells were cultured in a humidity-controlled environment under 5% CO2 at 37 °C, using Minimum Essential Medium α (Gibco) supplemented with 10% fetal bovine serum (HyClone), 100 units/mL penicillin, and 100 μg/mL streptomycin (FUJIFILM Wako Pure Chemical Corporation).



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    Example images of a nuclear bleb in <t>HT1080</t> cells stably expressing NLS-GFP (green) and H2B-mCherry (gray). (A, B) Super plot nuclear bleb to body ratio (bleb/body) of NLS-GFP and H2B-mCherry (A) in cells in between nuclear ruptures, biological triplicates n = 22, and (B) in cells no nuclear rupture taken every hour, n = 5, 5, 3. Example images and super plots of nuclear bleb to body ratio for Hoechst 33342 DNA stain (cyan) and H3 (magenta) for cell lines and drug treatments (C) MEF WT n = 8, 15, 7, (D) MEF VPA n = 13, 11, 10, (E) HT1080 WT n = 10, (F) HT1080 VPA n = 8, 9, 13. White arrows denote the nuclear bleb. Mean ± s.e.m. is graphed. Statistical significance is denoted by *P<0.05, **P<0.01, ***P<0.001 or ns (not significant) via two-tailed paired Student’s t-test. Scale bars: 10 µm.
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    Example images of a nuclear bleb in <t>HT1080</t> cells stably expressing NLS-GFP (green) and H2B-mCherry (gray). (A, B) Super plot nuclear bleb to body ratio (bleb/body) of NLS-GFP and H2B-mCherry (A) in cells in between nuclear ruptures, biological triplicates n = 22, and (B) in cells no nuclear rupture taken every hour, n = 5, 5, 3. Example images and super plots of nuclear bleb to body ratio for Hoechst 33342 DNA stain (cyan) and H3 (magenta) for cell lines and drug treatments (C) MEF WT n = 8, 15, 7, (D) MEF VPA n = 13, 11, 10, (E) HT1080 WT n = 10, (F) HT1080 VPA n = 8, 9, 13. White arrows denote the nuclear bleb. Mean ± s.e.m. is graphed. Statistical significance is denoted by *P<0.05, **P<0.01, ***P<0.001 or ns (not significant) via two-tailed paired Student’s t-test. Scale bars: 10 µm.
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    Image Search Results


    Example images of a nuclear bleb in HT1080 cells stably expressing NLS-GFP (green) and H2B-mCherry (gray). (A, B) Super plot nuclear bleb to body ratio (bleb/body) of NLS-GFP and H2B-mCherry (A) in cells in between nuclear ruptures, biological triplicates n = 22, and (B) in cells no nuclear rupture taken every hour, n = 5, 5, 3. Example images and super plots of nuclear bleb to body ratio for Hoechst 33342 DNA stain (cyan) and H3 (magenta) for cell lines and drug treatments (C) MEF WT n = 8, 15, 7, (D) MEF VPA n = 13, 11, 10, (E) HT1080 WT n = 10, (F) HT1080 VPA n = 8, 9, 13. White arrows denote the nuclear bleb. Mean ± s.e.m. is graphed. Statistical significance is denoted by *P<0.05, **P<0.01, ***P<0.001 or ns (not significant) via two-tailed paired Student’s t-test. Scale bars: 10 µm.

    Journal: bioRxiv

    Article Title: Nuclear blebs are composed of variable chromatin states but consistently enrich transcription initiation relative to elongation

    doi: 10.64898/2026.03.10.710873

    Figure Lengend Snippet: Example images of a nuclear bleb in HT1080 cells stably expressing NLS-GFP (green) and H2B-mCherry (gray). (A, B) Super plot nuclear bleb to body ratio (bleb/body) of NLS-GFP and H2B-mCherry (A) in cells in between nuclear ruptures, biological triplicates n = 22, and (B) in cells no nuclear rupture taken every hour, n = 5, 5, 3. Example images and super plots of nuclear bleb to body ratio for Hoechst 33342 DNA stain (cyan) and H3 (magenta) for cell lines and drug treatments (C) MEF WT n = 8, 15, 7, (D) MEF VPA n = 13, 11, 10, (E) HT1080 WT n = 10, (F) HT1080 VPA n = 8, 9, 13. White arrows denote the nuclear bleb. Mean ± s.e.m. is graphed. Statistical significance is denoted by *P<0.05, **P<0.01, ***P<0.001 or ns (not significant) via two-tailed paired Student’s t-test. Scale bars: 10 µm.

    Article Snippet: Human fibrosarcoma HT1080 cells obtained from the American Tissue Culture Collection (ATCC) were cultured and passaged similarly.

    Techniques: Stable Transfection, Expressing, Staining, Two Tailed Test

    Examples of nuclear blebs in (A) MEF cells, (B) human HT1080 cells, and (C) human prostate cancer cell lines imaged via Hoechst (DNA) and euchromatin markers H3K27ac and H3K9ac. Super plots of nuclear bleb to body ratios biological triplicates in (A) MEF and (B) HT100 wild type (WT), increased euchromatin (VPA), decreased heterochromatin (DZNep), and lamin A knockdown (LA KD, MEF only). (C) Super plots of nuclear bleb to body ratios in human prostate cancer cell lines LNCaP n = 33, PC3 n = 30, and DU145 n = 29. Mean ± s.e.m. is graphed in A, B, and C. Statistical significance is denoted by * P <0.05, ** P <0.01, *** P <0.001 or ns (not significant) via One way Anova with post-hoc Turkey test. Scale bars: 10 µm.

    Journal: bioRxiv

    Article Title: Nuclear blebs are composed of variable chromatin states but consistently enrich transcription initiation relative to elongation

    doi: 10.64898/2026.03.10.710873

    Figure Lengend Snippet: Examples of nuclear blebs in (A) MEF cells, (B) human HT1080 cells, and (C) human prostate cancer cell lines imaged via Hoechst (DNA) and euchromatin markers H3K27ac and H3K9ac. Super plots of nuclear bleb to body ratios biological triplicates in (A) MEF and (B) HT100 wild type (WT), increased euchromatin (VPA), decreased heterochromatin (DZNep), and lamin A knockdown (LA KD, MEF only). (C) Super plots of nuclear bleb to body ratios in human prostate cancer cell lines LNCaP n = 33, PC3 n = 30, and DU145 n = 29. Mean ± s.e.m. is graphed in A, B, and C. Statistical significance is denoted by * P <0.05, ** P <0.01, *** P <0.001 or ns (not significant) via One way Anova with post-hoc Turkey test. Scale bars: 10 µm.

    Article Snippet: Human fibrosarcoma HT1080 cells obtained from the American Tissue Culture Collection (ATCC) were cultured and passaged similarly.

    Techniques: Knockdown

    Examples of nuclear blebs in (A) MEF cells, (B) human HT1080 cells, and (C) human prostate cancer cell lines imaged via Hoechst (DNA) and heterochromatin markers H3K27me3 and H3K9me2,3. Super plots of nuclear bleb to body ratios biological triplicates in (A) MEF and (B) HT100 wild type (WT), increased euchromatin (VPA), decreased heterochromatin (DZNep), and lamin A knockdown (LA KD, MEF only). (C) Super plots of nuclear bleb to body ratios in human prostate cancer cell lines LNCaP n = 10, 10, 9, PC3 n = 11, 11, 11, and DU145 n = 24. Mean ± s.e.m. is graphed in A, B, and C. Statistical significance is denoted by * P <0.05, ** P <0.01, *** P <0.001 or ns (not significant) via One way Anova with post-hoc Turkey test. Scale bars: 10 µm.

    Journal: bioRxiv

    Article Title: Nuclear blebs are composed of variable chromatin states but consistently enrich transcription initiation relative to elongation

    doi: 10.64898/2026.03.10.710873

    Figure Lengend Snippet: Examples of nuclear blebs in (A) MEF cells, (B) human HT1080 cells, and (C) human prostate cancer cell lines imaged via Hoechst (DNA) and heterochromatin markers H3K27me3 and H3K9me2,3. Super plots of nuclear bleb to body ratios biological triplicates in (A) MEF and (B) HT100 wild type (WT), increased euchromatin (VPA), decreased heterochromatin (DZNep), and lamin A knockdown (LA KD, MEF only). (C) Super plots of nuclear bleb to body ratios in human prostate cancer cell lines LNCaP n = 10, 10, 9, PC3 n = 11, 11, 11, and DU145 n = 24. Mean ± s.e.m. is graphed in A, B, and C. Statistical significance is denoted by * P <0.05, ** P <0.01, *** P <0.001 or ns (not significant) via One way Anova with post-hoc Turkey test. Scale bars: 10 µm.

    Article Snippet: Human fibrosarcoma HT1080 cells obtained from the American Tissue Culture Collection (ATCC) were cultured and passaged similarly.

    Techniques: Knockdown

    Example images and super plots of nuclear bleb to body ratio for DNA (cyan) and RNA Pol II pSer 2 (gray, elongation) and pSer5 (magenta, initiation) for cell lines (A) MEF n = 18, (B) HT1080 n = 10, (C) LNCaP n = 6, 10, 10, (D), PC3 n = 9, 7, 13, (E ) DU145 n = 12, 11, 11. (F) Graph summarizing all cell lines show a significant enrichment of RNA Pol II pSer5 relative to pSer2 in the nuclear bleb to body ratio. Mean ± s.e.m. is graphed. Statistical significance between pSer2 and pSer5 is denoted by * P <0.05, ** P <0.01, *** P <0.001 or ns (not significant) via two-tailed paired Student’s t -test. Scale bars: 10 µm.

    Journal: bioRxiv

    Article Title: Nuclear blebs are composed of variable chromatin states but consistently enrich transcription initiation relative to elongation

    doi: 10.64898/2026.03.10.710873

    Figure Lengend Snippet: Example images and super plots of nuclear bleb to body ratio for DNA (cyan) and RNA Pol II pSer 2 (gray, elongation) and pSer5 (magenta, initiation) for cell lines (A) MEF n = 18, (B) HT1080 n = 10, (C) LNCaP n = 6, 10, 10, (D), PC3 n = 9, 7, 13, (E ) DU145 n = 12, 11, 11. (F) Graph summarizing all cell lines show a significant enrichment of RNA Pol II pSer5 relative to pSer2 in the nuclear bleb to body ratio. Mean ± s.e.m. is graphed. Statistical significance between pSer2 and pSer5 is denoted by * P <0.05, ** P <0.01, *** P <0.001 or ns (not significant) via two-tailed paired Student’s t -test. Scale bars: 10 µm.

    Article Snippet: Human fibrosarcoma HT1080 cells obtained from the American Tissue Culture Collection (ATCC) were cultured and passaged similarly.

    Techniques: Two Tailed Test